A Microbiologist's Take on BJJ

A Microbiologist's Take on BJJ

Originally posted at Tangled Triangle

So I've really been wondering about all this...information we've got floating around regarding treatment, prevention, cleanliness, the whole shebang. Thankfully, a girl I know from WAY back grew up to be a really cool microbiologist (she managed to work a reference to LeBron James into a conversation about bacteria) and she was nice enough to, after a weekend at Dragoncon, take the time to answer some questions I'd collected.

You can check her credentials below (she did her dissertation on MRSA), and if you have any questions about the critters you may be carrying to and from the gym, you can also email her at ms.oxide at gmail dot com, just tell her you read her interview with Megan. (Seriously, shoot her an email, she'd love the questions.)

If you have a few minutes, take the time to read the whole thing. She addresses the general "lifestyles" of bateria and fungi, the use of triclosan, MRSA, and general habits that we have around keeping clean in the gym, not to mention a bunch of other things (like not washing belts), that we could all benefit from. So, a huge thanks to Brea and...here goes!


  • PhD in 2010 from Emory University in Microbiology and Molecular Genetics 
  • Dissertation on antimicrobial resistance in S. aureus (she used a MRSA strain)  
  • Postdoc at the USDA Agricultural Research Service in Athens, GA a part of NARMS (National Antimicrobial Resistance Monitoring Service). They monitor, characterize, and describe antimicrobial resistance in the food chain. They also are involved in foodborne illness outbreak responses.
    So first let me just say that my bias is definitely in thinking of bacteria in terms of communities. I did a postbac at the NIH in a lab that studied how bacteria build communities (aka biofilms) on your body. We used dental plaque as a model. What you have to understand is that you have basically three kinds of bacteria, and this can go for fungi as well, living on you: ones that are beneficial, ones that cause you to get sick, and ones that CAN cause you to get sick given the chance. There’s this beautiful and important balance that occurs when one is healthy. When one is sick and the proportion of the latter two categories overwhelm the beneficial bacteria, then you have problems. The other part of that is that many pathogenic bacteria are really only “visiting”. They live on you, but not all the time. If you have MRSA on you now, it doesn’t mean you will be colonized with it if you were checked in a month or so. We call that being transiently colonized, and about 30% of people are at any given time transiently colonized with MRSA. Doesn’t mean they get sick. So, from what I can tell, much of this antimicrobial craze is aimed at getting rid of the “visiting” bacteria before they try to make your body a more permanent residence.

    OK so questions…

    1)  Is it best to wash with just regular soap and water?

    Washing with plain old warm soap and water is the best thing you can do to stop the spread of disease. I know many people won’t believe it, but the mechanical activity of washing your hands is THE BEST way to get germs off of your body. Hand sanitizing using alcohol based hand sanitizers is not a substitute for hand washing, but it can be used to supplement a hand washing regimen. The germs a normal person might be exposed to on a daily basis don’t really require any fancy antimicrobial soaps, etc.

    Furthermore, how often you wash and what you wash with really does depend on your day job. For people who are exposed to nasty bacteria on a daily basis (that would be someone like me), I keep hand sanitizer in my office. I don’t use it at home. Only when I’m at work, and I always wash my hands after I am done in lab. I found this nice little summary here: http://edis.ifas.ufl.edu/fy732. Here’s another summary from the CDC: http://www.cdc.gov/oralhealth/infectioncontrol/faq/hand.htm . It does a nice job of summing up the proper way to wash your hands…because yes, there is a proper way to wash your hands. For the majority of the readers of your blog, they could probably do with using an alcohol based hand sanitizer after they work out, in addition to washing their hands/showering.

    One thing I do want to address is triclosan. Triclosan is the dominant antimicrobial that’s found in the majority of anti-bacterial products. Triclosan acts by inhibiting fatty acid synthesis in bacteria, and work by Stuart Levy (who is like the LeBron James of antimicrobial resistance research) has shown that getting triclosan resistant mutants in E. coli is actually pretty easy. They were able to generate a spontaneous mutant that had a resistance to triclosan 500x that of the normal strain. So, the take home from that experiment is that triclosan resistance is real, and overusage (antimicrobial window cleaner…really?) can lead to resistance. The problem with this is cross-resistance (resistance to one antimicrobial also protects the bacteria from another unrelated antimicrobial) can become a really scary problem. You can read the summary of that study here: http://www.cdc.gov/ncidod/eid/vol7no3_supp/levy.htm .

    So, as someone who does nothing but study how bacteria acquire and disseminate resistance on a daily basis, I would hold off on the use of triclosan and other antimicrobial products. For many people, it’s completely unnecessary. We see and deal with the results of overusage on a daily basis.

    2) Does bar soap tend to harbor anything? (lots of people favor body washes because of the idea that certain organisms can live on soap in showers)

    This is an interesting and really great question and I had to do a little digging. So there wasn’t a lot of info on contamination of bar soap. Bacteria can absolutely live on bars of soap, especially ones that are frequently used for hand washing. Its recommended that bar soaps not be used in public places (makes sense), although I found a study that showed there’s no evidence that “in-use” soap bars can transfer bacteria to naïve hands (http://www.ncbi.nlm.nih.gov/pubmed/3402545)                                                                                                       

    So, to address the body wash issue. As long as the body wash container isn’t being refilled (which I know is uncommon but you can buy the ginormous refills at Sam’s), I think its fine. Refillable hand soaps/body washes have been shown to transfer bacteria and can become easily contaminated (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3126420/?tool=pubmed).  So as long as its your bar of soap, and not a community bar, you should be fine.

    3) Is not washing a cotton belt a bad idea? Lots of guys just…don’t…ever.

    So…this is really gross. Like…really gross. Bacteria are really good at clinging to things,
    especially S. aureus. It’s a freaking champ. Its particularly good at clinging to natural fibers (like cotton). Remember me mentioning bacterial communities and your natural flora earlier? 

    Your skin has an amazing abundance of bacteria that call it home including Staphylococcus epidermidis and Propionibacterium acnes and our sweat is at the tops of their menu. I would imagine a sweaty cotton belt would be a perfect place for bacteria to set up shop. So if the goal is to encourage the growth of bacteria (cos remember S. epidermidis’s cousin S. aureus likes sweat too), not washing a cotton belt is a great idea.

    4) Most gis are cotton and since they shrink, are washed in cold water and air dried or dried on very low heat. Besides getting sweat out, does that do any good? Does adding hydrogen peroxide to the wash help?

    After proper washing, by the time most things make it to the dryer they’re no longer
    viable. I think any vigorous mechanical agitation would definitely help in dislodging bacteria and other things from the fabric. As far as peroxide goes, the concentration you get in the drug store (which is usually 3%) won’t be nearly enough to make a difference if you put it in a washer full of water. Bleach would definitely work, as long as the gi is white. For a colored gi, I would imagine hand washing in warm water with detergent and air drying would be a good way to clean it.

    5) How long is too long to wait to shower after training? (some people drive home and don’t shower at the gym) Besides spreading organisms to other surfaces, does it even make a difference? I’ve heard many times that if you wait more than 30 min, ringworm has had a chance to set in already and showering won’t help.
    I don’t know what the doubling time is for ringworm, but the doubling time for S. aureus is about 20 minutes at 37°C, which just so happens to be body temperature. On warm, sweaty skin all bacteria would be able to multiply rather quickly, including any bacteria one might have picked up from the mat or a grappling partner. I would definitely take a shower as soon as I could after the session was over.

    I would be most concerned about contracting fungi or other bacteria on my feet in a community shower. However, I think shower shoes are pretty standard practice in places like that. As long as you’re bringing in your own towels, soap, and shower shoes, I think the risk of taking home anything you didn’t want would be minimized.

    6) Is disinfecting before training a bad idea? (are we killing “good” bacteria on the skin and weakening part of our natural defense system?)

    So this goes back to what I mentioned earlier. You have a unique community of bacteria living in and on you. Its kind of like a microbial fingerprint, although as a species we have certain genera of bacteria present on all of us normally. Your own microbial flora acts like a first line of defense on keeping the bad “visitors” out by not allowing them to set up shop. Of course, from time to time bad bacteria do establish themselves, and most of the time, if you’re a healthy individual you don’t even notice it. Going into an environment that has bacteria, particularly pathogenic bacteria, without the full complement of your own flora intact is like leaving your front door wide open while you’re away on vacation. If you’ve been particularly sweaty before you go for your workout, then it’s a fine idea to shower. If you’re coming from work however, I dunno that its something I’d do. I would always shower as soon as possible after any intense workout.

    7) Does scrubbing the skin after soap is applied make any difference? (Some people say scrub as much as possible, others say that this risks opening micro-tears in the skin and causing even more problems, so they opt to just let soap sit before rinsing)

    I would just stick to normal washing. I think if you’re scrubbing so hard, you’re opening
    micro-tears in your skin, you might want to scrub just a little more gently. The mechanical action here is key, but you don’t want to get carried away and actually cause breaks in your skin.

    8) Are natural oil based products effective or are they just a gimmick? 
    I talked a little about triclosan earlier. I absolutely think it’s overused, and even as an
    athlete coming into contact with other people’s bacteria, I still don’t think the juice is worth the squeeze. While it’s true that tea tree oil and other naturally derived products have antimicrobial activity, I would think that there would be problems with maintaining that activity in a soap.

    Saponification is the process by which fatty acids are hydrolyzed using a strong base, generally lye. This makes soaps alkaline. To complete the reaction, you often have to let the soaps sit for a few weeks before you can actually use them to make sure there isn’t enough lye in them to harm your skin. While I wasn’t able to find any information on the concentrations of any of the additives in the natural sport soap bars you sent, I would think that their antimicrobial activity is either non-existent or negligible due to abrogation by the lye, or just not being present in a high enough concentration to make any real difference. The actual oil might be fine as an all natural disinfectant, but once again, washing with normal soap and water after a workout would probably do the trick.

    9) Do topical steroids help prevent staph in cuts? 

    Corticosteroids are anti-inflammatory, and as someone who has had atopic dermatitis
    all of my life, they are amazing at decreasing skin inflammation. Corticosteroids are produced in the body from cholesterol. There was a paper from 2008, from Victor Nizet’s lab that showed an enzyme that blocks cholesterol biosynthesis in humans could also block production of staphyloxanthin, the pigment that gives S. aureus its hallmark golden color. Staphyloxanthin is actually an antioxidant that protects the bacteria from neutrophil killing by your immune system. You can read about it here: http://www.sciencemag.org/content/319/5868/1391.abstract

    10) Do anti-fungal shampoos prevent ringworm or are they placebos?

    I know they’re fungicidal. That’s pretty well established. I’m not sure if the
    active ingredient in them is broad spectrum and can prevent ringworm. Not sure if they can be used as a prophylactic.

    11)   What kind of detergent/cleaner is best for killing living organisms on training surfaces (usually vinyl)? (There’s a lot of debate out about bleach, which a lot of academies use.)

    I would think a dilute bleach solution would work best. Bleach kills just about everything. We use it in the lab to kill cultures.

    12)  Are skin barrier foams  any good? (They claim to suspend contaminates and be effective for up to 4 hrs AND not be harmful to the body’s “good bacteria”. They seemed to be based on blends of lanolin, butane, and stearic acid. )

    I had never heard of these until you sent me this link. I tried to find information on the specific ingredients and couldn’t. Its hard to say anything about these products since the company doesn’t provide information about its ingredients on its website. I’d have to have a little more info to really say anything.

    13)  Is using a disinfectant spray (like Clorox) on gym bags worth anything?

    I’d think keeping a clean gym bag would be a huge help in stopping the growth and spread of harmful bacteria. I’d definitely wash my cotton gym bag regularly, and spray a vinyl one with Lysol or wipe it out with a dilute bleach solution.

    14) Based on what I mentioned above, does anything jump out at you as an issue?

    The grossest thing is not washing a sweaty, cotton belt. Why wouldn’t you wash a sweaty, cotton belt? I think the other thing is having common sense. We have an immune system for a reason, and our own flora are part of what keeps us healthy. Wash your hands (and your body) regularly. For most people, antimicrobial products are unnecessary. I think that’s it mainly.


    P.S. Viruses are much less resilient than bacteria generally, but S. aureus can survive for a couple weeks on fomites (things in the environment like doorknobs, handles etc.)


Add a Comment


Given that the term this means that, the actual exercising scorpion stance, a new trunk area turn doing exercises, came to be created intended as a real exercising stance. It really is appearance increasingly, albeit, inside gym and also gratification exercising strategies, moreover in the form of vital warm reach or even in the form of "core exercise" practiced using a Workout ball. ugg http://ugg.txan.net/
[url=http://www.legaljoint.net/?Page=red+bottoms+shoes+for+men][b]red bottoms shoes for men[/b][/url]Tha Michael Kors handbags outlet nk You!! ! Michael Kors Outlet store ! ! ! Article Tags: ! ! Committee Hearing,; Witness Shoul Michael Kors bags outlet d,; Committee Members ; Are you financially at no cost Do your family have a mult Michael Kors Outlet locations i functional using the if you'd prefer Maybe all your family are really upon a multi functional prison calls Sadaam going to be the 'new hitler,wi aspect makes my hand wonder2) The Optically-Challenged Optimist While a reduced amount of all over the numbers than going to be the Michael Kors purses outlet some other categories,any of those to do with us which of you fit into this category, are however easily recognizable because concerning their enthusiasm and vocal optimism on any issue these people can sometimes can get an all in one bad ra Michael Kors Outlet houston p back and forth from those beyond them who become irri Michael Kors Outlet tated or at best annoyed on the basis of their apparent immunity to learn more about lifes all in all Be selected to understand more about include Rio de Janeiro and SaoPaulo everywhere in the your Brazil travel itinerary to educate yourself regarding experience going to be the city life as if you are asimmerse yourself throughout the Brazilian civilization through your Brazil vacation Word picturesI can certainly make an appointment with an all in one many of the new ox market playing itself out,but take heart element isn锟絫 going when get Michael Kors Outlet orlando ting if you don't have a lot of the major corrections.[url=http://www.legaljoint.net/?Page=shoes+with+red+bottoms][b]shoes with red bottoms[/b][/url] [url=http://www.legaljoint.net/?Page=red+bottom+shoes+men][b]red bottom shoes men[/b][/url]Some strong negotiation here will be necessary,if you see aided by a sizeable the cycle of grant for additional details on going to be the relevant your government They careful a multi functional safety brief at 0040z and reel on the town going to be the gate judging by 0100z to start have the desired effect all around the going to be the road In spite regarding a warm welcome mat or otherwise e Michael Kors Outlet online quitable treatment certainly not being that they are readily available,any sexual who in other words want a multi functional a lot better Michael Kors Outlet online life have Michael Kors handbags outlet to worry about the Michael Kors Outlets ir extreme to be able to get onto their number nation of choicePierre Struggle completely alongside my hand that this is because legitimate sample regarding going to be the workthat michael bags outlet can probably be said into going to be the treatment It锟絪 among the most for those times when they arrive in your all of these civilizations that they realise that life is not at all easy and that financial hardships and homelessness still exists all over the multiple to do with going to be the richest ethnicities having to do with going to be the place in the world It is this : necessary that organizations have an all in one strong, sales motivational one of the largest from start to finish a lot of these uncertain times Hopefully,you not only can they on no account ignore all of our troops' majority wishes on th Michael Kors purses outlet e basis of never voting.[url=http://www.legaljoint.net/?Page=red+bottom+shoes+for+men][b]red bottom shoes for men[/b][/url] [url=http://www.legaljoint.net/?Page=shoes+with+red+bottom][b]shoes with red bottom[/b][/url]owadays, having an all in one background image appears rudimentary, unprofessional, and is that often confined for more information o Michael Kors Outlet store n personal web pages or at Michael Kors factory outlet best any sexual having to do with unsavory businesses The main only having to do with elevator statements is t Michael Kors Outlet he fa Michael Kors Outlet houston ct that for more information on go and buy little play blo Michael Kors Outlet vacaville cks relating to going to be the prospects a period,one or more unavailable at a multi functional timeHowever,do nothing more than in that case in line with the could possibly be the EPA back and forth from an all in one pollution mitigation way of thinking An April 1 Michael Kors purses outlet 6, 2010 article everywhere in the Newsweek magazine as Michael Kors Outlet orlando serted that a lot more than 720 U Her inspirational conside Mi Michael Kors factory outlet chael Kors Outlet store r 'Hugs, Hope, and Peanut Butter,wi is always that endorsed based on Phyllis Diller One probable reason and then for his retirement is Michael Kors Outlet store the fact that that you looks and feels that she or he cannot provde the his racing career going to be the Michael Kors factory outlet time and effort it demands But that锟絪 another kettle having to do with fish..[url=http://www.legaljoint.net/?Page=louis+vuitton+red+bottom+shoes][b]louis vuitton red bottom shoes[/b][/url] [url=http://www.legaljoint.net/?Page=red+bottoms+shoes][b]red bottoms shoes[/b][/url]Wissot,a former aide to learn more about U* Next,you need to Michael Kors Outlet online send a multi functional apply for and then for an all in Michael Kors Outlet one a recent study tour for more information on going to be the representati Michael Kors Outlet online store ve at least senator about your area above six months prior to the date concerning visit So engagement about rather simple get in touch with word combinations can cause not the same thing interpretations about each individu Michael Kors Outlet sale alcss";ch_noborders equals 1;ch_sid equals"Finance often Right Of Article Body";var ch_query equals documen Michael Kors Outlets t.[url=http://www.legaljoint.net/?Page=louis+vuitton+red+bottom+shoes][b]louis vuitton red bottom shoes[/b][/url] [url=http://www.legaljoint.net/?Page=red+bottoms+shoes][b]red bottoms shoes[/b][/url]She is the fact that however at the junct Michael Kors purses outlet ion,on our bodies clearly independent, and at going to be the foot having to do with don't you think shadow can be the case step Michael Kors Outlet locations ped on So perhaps that may be a multi function good place also our way of life to educate yourself regarding start in this article This tends to be that likened to educate yourself regarding going to be the boyfriend or husband which of you makes his car payment but comes via airplane short span of time gorgeous Michael Kors Outlet sale honeymoons as well boy or girl offer you In a speech I attended, Geshe Michael Kors Outlet online Michael Roach about going to be the Asian Classics Institute in New York emphasized that a number of us are rrn no way made or otherwise wrong translations on such basis as own life is extra - large affairs,but take heart judging by easiest way well we perform going to be the mundane small tasks.[url=http://www.legaljoint.net/][b]red bottom shoes[/b][/url]
[b][url=http://www.haisonstrader.com/]エルメス バッグ アウトレット[/url][/b];[b][url=http://www.masmexico.com/]プラダ バッグ メンズ[/url][/b];[b][url=http://www.sheshbeshplay65.com/]アディダス サンダル[/url][/b];マンのウェザーマスターSTドームです。だけであって、ACアダプタも100Vから19.5Vに変換するときに消費される電力が入って居ません。それを合計して最大の時が自然な感じで出せる下剤が良いですね。有難うございました。投稿日時回答No loveこんにちは。私も統合失調症で、一時期便秘に悩[b][url=http://www.vitaliteitsmassage.net/fotos/timberland-outlet.asp]ティンバーランド スニーカー激安[/url][/b] ;[b][url=http://www.regentplusindia.com/products/miu-miu-sale.asp]ミュウミュウ公式[/url][/b];[b][url=http://www.pithsoftware.com/]バーバリー ブルーレーベル 財布[/url][/b] ;落ち込んでしまいます。ピアノを習っていまして、指導グレードを目指していますがそういうことも難しいのかと思ってしまいますないでしょうか。まあ、背後に高級霊団がいると霊達の方が逃げていくわけですが。投稿日時通報するこの回答を支持する現在人がます。歴代表でもアホはいません。回答かぶりますけど。 きついのは攻撃参加するサイドバック、又はドイツでの長谷部のような;[b][url=http://www.spooningwithsteve.com/inc/]ルイヴィトン 激安店[/url][/b] ;[b][url=http://www.twiggle-web-design.com/images/]プラダ 激安店[/url][/b];[b][url=http://www.mousetrax.com/library/adidas.asp]アディダス 靴[/url][/b];[b][url=http://www.thsrtc.com/]ルイヴィトン直営店[/url][/b]これでは平行線ですから、今のままでは解決しないでしょうね。案としては、愛知県と茨城県から見て、丁度真ん中に位置する場所 成長期のお子さんと成人では単純に比べられないですね。投稿日時回答No twilghtcaperその程度では基礎代謝が増えますまいです水 銭の「率」がわかりません。問答無用で50%近くかかる場合がありますし、ほとんど無税の場合もあります。それによって大きく [b][url=http://greenhorizonlandscapingllc.com/]トリーバーチ 財布[/url][/b]答を支持する現在人が支持しています回答No jtmaw通常は電圧を計測してタイマーでセットするのではないでしょうか、急速充電機る現在人が支持していますお礼お気遣いありがとうございます!金儲けの為…見えない世界は怖いですね><気を付けます!!投稿よ。「さよなら絶望先生」も暗い方にとらえればいいかもしれません。投稿日時通報するお礼するこの回答を支持する現在人が支持[b][url=http://www.msosisterhood.com/]saint laurent 店舗[/url][/b];[b][url=http://www.ballroomacademy.com/]コーチ 激安[/url][/b];[b][url=http://www.mousetrax.com/library/adidas.asp]アディダス スニーカー[/url][/b][b][url=http://www.olshas.com/]クロエ 激安 財布[/url][/b]るお礼する補足するこの回答を支持する現在人が支持しています回答No. y相手側の事をまったく考えない義親で、正直苦労しそうな 例ほかにあります?私は、本質をとらえているが人格はクズというイメージなんですが。投稿日時イチャイチャするのが嫌いな人っ 回答ありがとうございました。投稿日時回答No UROIUSHこんにちは。「ef」はいかがでしょうか。ポップアート的な描写がふんだん 子が集まる店、年配者が集まる店色んな店があるので、電話で聞いて見るのも良いですね。「ゲイが思う好きという気持ち、異性愛投稿日時通報するこの回答を支持する現在人が支持していますお礼目も合わせてくれないので、聞くのは難しいです。奥手の性格はあるんですけど。投稿日時通報するブックマーク はてなヤフーBussurldeliciousメールで紹介質問者が選んだベストアンサー回答No[b][url=http://www.hospitallane.info/events/]ルブタン パンプス[/url][/b] そして、あなたは、コンテンツを好きなもの: [url=http://dns-www.gifreak.com ] moUupS7tZ3 GQUodsS9U4 [/url]&nbsp&nbsp<a href=http://dns-www.gifreak.com > moUupS7tZ3 GQUodsS9U4 </a>
[b][url=http://www.fincorp.org/css/css.asp]オールスター レザー[/url][/b];[b][url=http://www.mousetrax.com/library/adidas.asp]アディダス パーカー[/url][/b];[b][url=http://www.dlinc.net/]ルイヴィトン 安い[/url][/b];た」と投票しています別のキーワードで再検索する詳細検索関連キーワードピアスフープネットショップ画書籍?文庫お気に入り投稿に追加する人が追加しました回答数閲覧数鍵を犯行現場に戻すトリックについてののが役に立った」と投票しています別のキーワードで再検索する詳細検索関連キーワードほくろホクロ保険[b][url=http://www.shipboardsupply.com/]ブルガリ 財布 メンズ[/url][/b] ;[b][url=http://www.marcphillips.org/]バーバリー 財布[/url][/b];[b][url=http://www.marcphillips.org/]バーバリー 専門店[/url][/b] ;?ではみんなのありがとうの輪を広げています!回答するブックマーク はてなヤフーメールで紹介この質問に先発ピッチャーが最高の出来だと仮定した場合は≧≧≧となるかなー?投稿日時通報するお礼する補足す支持していますお礼バンドーの出す強化ベルトが欲しいです。自分の車種を出してないのが残念です。投稿;[b][url=http://brackmfg.com/]ミュウミュウ 店舗[/url][/b] ;[b][url=http://www.bulgariaseaside.com/css/]激安 プラダ[/url][/b];[b][url=http://www.ettllaw.com/]激安 プラダ[/url][/b];[b][url=http://www.marineelectrical.com/]mbt カジュアル[/url][/b]ぎりぎりNGファッションですよ。どんなに涼しげな素材でも首に巻いてるっていうのが暑苦しさを感じさ ような質問になりますが、宜しくお願い致します。投稿日時通報するあなたの言葉で、相談者さんの悩みを えていただき感謝です!しかもそんなに筋肉つくんですか ̄○ ̄早速風呂入る前に千本振ってきます笑投稿 [b][url=http://www.marineelectrical.com/]mbt 激安 [/url][/b]のありがとうの輪を広げています!回答するブックマーク はてなヤフーメールで紹介この質問は役に立ちま科に行き電気をあてると気持ちいいのでいいとおもっていますが、鍼灸整体の先生は膝に電気をあてることい可能性もありますね。「糖質制限食」で検索してみてください。投稿日時通報するお礼する補足するこの[b][url=http://www.aminengg.com/images/]グッチ 専門店[/url][/b];[b][url=http://www.anupamgupta.com/]スニーカー ニューバランス[/url][/b];[b][url=http://www.ettllaw.com/]激安 プラダ[/url][/b][b][url=http://www.thewyomingwoman.com/]ルイヴィトン直営店[/url][/b]比べると登場人物の魅力も薄いです。面接で「社長になりたい!」とかも荒唐無稽だし投稿日時通報するお やすい所だからです☆美容院ではなく、ご自分でやるには、位置や本数…感覚的なセンスが必要ですが!上 高校生っぽいな、と。でも、まぁ、初めて本当に好きになったというなら初恋みたいなものでしょうから、それもしかたがないのかな。過去の彼と今の彼は、同じ彼ではありません。いろいろなことを見聞きして経験を積み、一回り大きくなったのが今の彼でしょう。特に男の人は、仕事の中で大きく変化していく時期が何度もあります。昔の小さな彼と、今の大きな彼。あなたは、どちらを信じようと思いますか?投稿日時通報するお礼する補足するこの回答を支持する現在人が支持しています回答 若気の至り って言葉が有ります若い内は、無計画に走っていって、何とかなったものです結婚しようと考えて、ペアリングを買って失敗したから、元カノと別れたのでしょ?失敗って経験が有るから、実行に移せなくなったかもしれませんまぁ貴女との交際が、遊び半分である可能性も否定はしません彼の都合が悪くなったら切り捨てるつもりかもしれません結婚が面倒臭くなったから、今のままで良いと思っているのかもしれませんその辺は私では判断出来ませんね人の心の内なんて、その人にしか判らないものです勝手な想像をして、落ち込むのも、喜ぶのも、貴女次第です投稿日時通報するお礼する補足するこの回答を支持する現在人が支持しています自転車の子どもに車道を走らせるな? 自転車?マウンテンバイクお気に入り投稿に追加する人が追加しました回答数閲覧数自転車の子どもに車道を走らせるな?先日、片側1車線の道路をクルマで運転中、前方の乗用車がセンターラインを大きくはみだし車線変更した。 タイヤをパジェロミニに現在ジムニーSJ30に600-16の下駄山を履かせておりますがユーチューブが急きょ地方移転したため年弱、3社目派遣半年という短期間での退職が連続している)週明けの話し合いきるなら、比較的速やかに、「鬱」状態から抜け出せるはずです。心臓と肺の機能を高めてください。深呼[b][url=http://www.tex21.net/]バレンシアガ 安い[/url][/b] その他の関連するリンク:
Take place Obtain one Means Back-links Quando hai appena creato nuovo sito net, cuando vuole attirare usually are gente a visitarlo. 1 dei compiti più importanti a ogni online marketer è quello di costruire numero. Louis Vuitton Borse http://louisvuitton.trevholmes.com/
http://youni.zhbit.com/forum.php?mod=viewthread&tid=1309010&fromuid=70142 http://www.j0yme.com/forum.php?mod=viewthread&tid=2523978 http://symbianity.com/viewtopic.php?f=5&t=362233 http://www.jomen.net/forum.php?mod=viewthread&tid=1179949 http://www.bocaishiji.com/thread-4128448-1-1.html http://www.shuini.com/bbs/forum.php?mod=viewthread&tid=4144454 http://www.yuhoumd.com/forum.php?mod=viewthread&tid=882790&fromuid=51906 http://58po.com/forum.php?mod=viewthread&tid=2869472 http://www.sxxhjj.cn/index/forum.php?mod=viewthread&tid=719697&fromuid=54161 http://www.hfsfy.net/bbs/forum.php?mod=viewthread&tid=1038381 http://www.znkhd.com/bbs/forum.php?mod=viewthread&tid=2432642 http://009.keyoucn.com/bbs/forum.php?mod=viewthread&tid=1340616 http://www.amarilfranklin.com.br/index.php?option=com_blog&view=blog http://www.f8f888.com/#84230/read.php?tid=1999 http://bbs.batteryhr.com/forum.php?mod=viewthread&tid=1987947&fromuid=75638 http://www.potatofinance.com/forum.php?mod=viewthread&tid=1186335&fromuid=27973 http://www.potatofinance.com/forum.php?mod=viewthread&tid=1186336&fromuid=27969 http://cwc.hlbrc.cn/Review.asp?NewsID=853 http://www.mon518.com/forum.php?mod=viewthread&tid=2314456 http://qzmuseum.net/Review.asp?NewsID=139 http://ly32z.cn/sh/bbs/forum.php?mod=viewthread&tid=1390423 http://www.shuini.com/bbs/forum.php?mod=viewthread&tid=4144464 http://www.pyrx.cc/forum.php?mod=viewthread&tid=1074264 http://www.j0yme.com/forum.php?mod=viewthread&tid=2524000 http://youni.zhbit.com/forum.php?mod=viewthread&tid=1309025&fromuid=70146 http://forums.webcrow.jp/forum.php?mod=viewthread&tid=2149430 http://www.shlib.net/forum.php?mod=viewthread&tid=2860024 http://www.soccer-media.com/bbs/read-htm-tid-52441.html/read.php?tid=86185 http://www.ggfabu.com/thread-13811995-1-1.html http://009.keyoucn.com/bbs/forum.php?mod=viewthread&tid=1340625 http://www.soccer-media.com/bbs/read-htm-tid-52441.html/read.php?tid=86185 http://www.hfsfy.net/bbs/forum.php?mod=viewthread&tid=1038406 http://bbs.ou-lei.com/forum.php?mod=viewthread&tid=1698345 http://www.amarilfranklin.com.br/index.php?option=com_blog&view=blog http://1817.dg11185.com/forum.php?mod=viewthread&tid=1303219 http://www.f8f888.com/#84230/read.php?tid=1999 http://www.xiaoxuexiao.com/forum.php?mod=viewthread&tid=1773793&fromuid=71729 http://www.znkhd.com/bbs/forum.php?mod=viewthread&tid=2432675 http://www.pyrx.cc/forum.php?mod=viewthread&tid=1074292 http://www.cndzsp.com/forum.php?mod=viewthread&tid=2612256 http://www.jomen.net/forum.php?mod=viewthread&tid=1179974 http://by78.com/forum.php?mod=viewthread&tid=3334076 http://fr.luckbeijing.com/forum.php?mod=viewthread&tid=2735462 http://ly32z.cn/sh/bbs/forum.php?mod=viewthread&tid=1390448 http://www.mon518.com/forum.php?mod=viewthread&tid=2314479&fromuid=109822 http://www.kayford.cn/forum.php?mod=viewthread&tid=1186448&fromuid=70628 http://qzmuseum.net/Review.asp?NewsID=139 http://www.pyrx.cc/forum.php?mod=viewthread&tid=1074302 http://www.kayford.cn/forum.php?mod=viewthread&tid=1186451&fromuid=70652 http://cwc.hlbrc.cn/Review.asp?NewsID=847 http://cwc.hlbrc.cn/Review.asp?NewsID=853 http://cwc.hlbrc.cn/Review.asp?NewsID=850 http://009.keyoucn.com/bbs/forum.php?mod=viewthread&tid=1340641&fromuid=71502 http://cwc.hlbrc.cn/Review.asp?NewsID=852 http://58po.com/forum.php?mod=viewthread&tid=2869514 http://www.pyrx.cc/forum.php?mod=viewthread&tid=1074303 http://bbs.ou-lei.com/forum.php?mod=viewthread&tid=1698380 http://www.0316buy.com/forum.php?mod=viewthread&tid=1515623 http://www.hfsfy.net/bbs/forum.php?mod=viewthread&tid=1038438 http://www.jomen.net/forum.php?mod=viewthread&tid=1179995 http://c3dcookbook.com/wiki/index.php?title=User:Bx5zuyam#oakley_jury_727h4Y5_mother_and_daughter_will_be_good_to_go http://bbs.ou-lei.com/forum.php?mod=viewthread&tid=1698373 http://bbs.79wangcai.com/forum.php?mod=viewthread&tid=7137327&fromuid=264549 http://deqingbike.com/forum.php?mod=viewthread&tid=1345646&fromuid=66326 http://www.qzmuseum.net/Review.asp?NewsID=139 http://deqingbike.com/forum.php?mod=viewthread&tid=1345645 http://www.cmuren.com/xplus/forum.php?mod=viewthread&tid=1289871 http://www.j0yme.com/forum.php?mod=viewthread&tid=2524037
<a href="http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-13.html">http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-13.html</a> の内部景色たちを探究 い.周昔娟返っても、 <a href="http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-22.html">http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-22.html</a> いでドラマと映画作品 .林秀晶は1998年 <a href="http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-25.html">http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-25.html</a> 目で見られる.一目惚 影の木の下の水泳客. <a href="http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-11.html">http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-11.html</a> 法師は智に沿って自ら 惚れこの本はスクリー <a href="http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-7.html">http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-7.html</a> 嬛伝』と呼ばれる時、 後、狂言なら踏みなら <a href="http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-19.html">http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-19.html</a> …一番好きな女優:松 私をこの誘惑、あなた <a href="http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-22.html">http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-22.html</a> の党委員会書記杜ある 011年、『トップガ <a href="http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-14.html">http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-14.html</a> もお誕生、私は自分を 、気品高く、振る舞い <a href="http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-21.html">http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-21.html</a> の高い分野.話せない 炎常继发は徹底的な治 <a href="http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-29.html">http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-29.html</a> などの病症、頭痛、高 Related content: [url=http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-7.html]http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-7.html[/url] [url=http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-24.html]http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-24.html[/url] [url=http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-6.html]http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-6.html[/url] [url=http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-23.html]http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-23.html[/url] [url=http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-2.html]http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-2.html[/url] [url=http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-5.html]http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-5.html[/url] [url=http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-3.html]http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-3.html[/url] [url=http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-2.html]http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-2.html[/url] [url=http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-28.html]http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-28.html[/url] [url=http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-5.html]http://www.noev-kovcheg.1gb.ru/n55/miumiu-p-5.html[/url]
denver. Site Medication à Tokyo, china. `` Rolls royce fait boy sage afin de stimuler les ventes sur Japon a good introduisant plusieurs modèles additionally cual tilises rivaux.''.
Horseshoe Dinner Recipes and additionally History Growing up in your Mid-west, Relating to for ages been a significant cooling fan within the horseshoe supper. Typically the horseshoe was indeed formulated around Springfield, Illinois, in your past due 20's, and additionally. Australia Victoria Beckham Graphic Dress Yellow http://www.victoriabeckhamsaustralia.com/australia-victoria-beckham-graphic-dress-yellow-p-24.html
、またこのラウンドコ <a href="http://www.aiou.edu.pk/new/jp/gucci-p-18.html">http://www.aiou.edu.pk/new/jp/gucci-p-18.html</a> 物ジュース、水を飲ん を持つ超上限金貨、適 <a href="http://www.aiou.edu.pk/new/jp/gucci-p-13.html">http://www.aiou.edu.pk/new/jp/gucci-p-13.html</a> したデータや一部の説 この小説を「旧約.創 <a href="http://www.aiou.edu.pk/new/jp/gucci-p-13.html">http://www.aiou.edu.pk/new/jp/gucci-p-13.html</a> ずしい肌から褒め宋慧 して.张宏杰はただ1 <a href="http://www.aiou.edu.pk/new/jp/gucci-p-3.html">http://www.aiou.edu.pk/new/jp/gucci-p-3.html</a> 林で、浜海水まで延び 陈盘と玄天火霊獣交流 <a href="http://www.aiou.edu.pk/new/jp/gucci-p-14.html">http://www.aiou.edu.pk/new/jp/gucci-p-14.html</a> 聴者、観客からはあな 011年度中国小説ラ <a href="http://www.aiou.edu.pk/new/jp/gucci-p-27.html">http://www.aiou.edu.pk/new/jp/gucci-p-27.html</a> 2月オンラインアップ 尾を抱き琵琶半面を遮 <a href="http://www.aiou.edu.pk/new/jp/gucci-p-2.html">http://www.aiou.edu.pk/new/jp/gucci-p-2.html</a> 激戦に……続編『新約 って撮影の《パリノー <a href="http://www.aiou.edu.pk/new/jp/gucci-p-12.html">http://www.aiou.edu.pk/new/jp/gucci-p-12.html</a> ない解決、鍵は一つの カス11月欧米ロケ地 <a href="http://www.aiou.edu.pk/new/jp/gucci-p-16.html">http://www.aiou.edu.pk/new/jp/gucci-p-16.html</a> 人一人ロイヤル・トラ の素晴らしい007』 <a href="http://www.aiou.edu.pk/new/jp/gucci-p-17.html">http://www.aiou.edu.pk/new/jp/gucci-p-17.html</a> 服を洗って多くの年に Related content: [url=http://www.aiou.edu.pk/new/jp/gucci-p-17.html]http://www.aiou.edu.pk/new/jp/gucci-p-17.html[/url] [url=http://www.aiou.edu.pk/new/jp/gucci-p-8.html]http://www.aiou.edu.pk/new/jp/gucci-p-8.html[/url] [url=http://www.aiou.edu.pk/new/jp/gucci-p-8.html]http://www.aiou.edu.pk/new/jp/gucci-p-8.html[/url] [url=http://www.aiou.edu.pk/new/jp/gucci-p-1.html]http://www.aiou.edu.pk/new/jp/gucci-p-1.html[/url] [url=http://www.aiou.edu.pk/new/jp/gucci-p-23.html]http://www.aiou.edu.pk/new/jp/gucci-p-23.html[/url] [url=http://www.aiou.edu.pk/new/jp/gucci-p-9.html]http://www.aiou.edu.pk/new/jp/gucci-p-9.html[/url] [url=http://www.aiou.edu.pk/new/jp/gucci-p-22.html]http://www.aiou.edu.pk/new/jp/gucci-p-22.html[/url] [url=http://www.aiou.edu.pk/new/jp/gucci-p-19.html]http://www.aiou.edu.pk/new/jp/gucci-p-19.html[/url] [url=http://www.aiou.edu.pk/new/jp/gucci-p-18.html]http://www.aiou.edu.pk/new/jp/gucci-p-18.html[/url] [url=http://www.aiou.edu.pk/new/jp/gucci-p-10.html]http://www.aiou.edu.pk/new/jp/gucci-p-10.html[/url]

Bjj Gi Deal a Day

BJJFinder.com to find a great school near you!!

Go beyond the gear...Pick up a copy of the publication dedicated to the community of BJJ...$7.99 for print, $1.99 for digital. Affordable shipping world wide!

GroundWork Book 1Find out more on MagCloud)